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human embryonic kidney (hek) 293ebna cells  (Thermo Fisher)


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    Structured Review

    Thermo Fisher human embryonic kidney (hek) 293ebna cells
    Human Embryonic Kidney (Hek) 293ebna Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+embryonic+kidney+(hek)+293ebna+cells/pm18308898-46-0-6
    Average 90 stars, based on 1 article reviews
    human embryonic kidney (hek) 293ebna cells - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Development of a mugineic acid family phytosiderophore analog as an iron fertilizer
    Article Snippet: HEK-293EBNA cells (Invitrogen, current: Thermo Fisher, Waltham, MA, USA) were cultured in Dulbecco’s modified Eagle’s medium (Nacalai Tesque, Kyoto, Japan) containing 10% fetal bovine serum (Gibco, Grand Island, NY, USA), 250 μg/mL geneticin (Phyto Technology Laboratories, Shawnee Mission, KS, USA) and 100 μg/mL gentamicin (Life Technologies, Carlsbad, CA, USA).

    Article Title: Me-talnetant and osanetant interact within overlapping but not identical binding pockets in the human tachykinin neurokinin 3 receptor transmembrane domains.
    Article Snippet: Recent clinical trials have indicated that neurokinin 3 receptor antagonists (S)-( )-N-{{3-[1-benzoyl-3-(3,4-dichlorophenyl)piperidin-3-yl]prop-1-yl}-4-phenylpiperidin-4-yl}-N-methylacetamine (SR142801; osanetant) and (S)-( )-N-( -ethylbenzyl)-3hydroxy-2-phenylquinoline-4-carboxamide (SB223412; talnetant) may treat symptoms of schizophrenia.. Using site-directed mutagenesis, rhodopsin-based modeling, [H](S)-( )-N-( ethylbenzyl)-3-methoxy-2-phenylquinoline-4-carboxamide (Me-talnetant) and [H]osanetant binding, and functional Schild analyses, we have demonstrated the important molecular determinants of neurokinin B (NKB), Me-talnetant, and osanetant binding pockets.. The residues Asn138, Asn142, Leu232, Tyr315, Phe342, and Met346 were found to be crucial for the NKB binding site.

    Cell Culture:

    Article Title: Clock gene BMAL2
    Article Snippet: .. Human embryonic kidney 293EBNA cells (Invitrogen) were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (Life Technologies) which cultured cells were then plated at 3×105 cells per well on six-well plates and transfected by using a total of 1.0 μg of various expression plasmids [an expression vector, plasmids containing reporter genes, 0.25 ng of Renilla luciferase reporter (pRL-CMV; Promega), and plasmids containing cDNA of each clock gene (cBmal1, cBmal2, cClock) with the amount indicated in FIG. 16] together with Lipofectamine plus (Life Technologies). .. As for the expression vector mentioned above, pcDNA3.1/V5/His empty vector (Invitrogen) was used.

    Modification:

    Article Title: Clock gene BMAL2
    Article Snippet: .. Human embryonic kidney 293EBNA cells (Invitrogen) were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (Life Technologies) which cultured cells were then plated at 3×105 cells per well on six-well plates and transfected by using a total of 1.0 μg of various expression plasmids [an expression vector, plasmids containing reporter genes, 0.25 ng of Renilla luciferase reporter (pRL-CMV; Promega), and plasmids containing cDNA of each clock gene (cBmal1, cBmal2, cClock) with the amount indicated in FIG. 16] together with Lipofectamine plus (Life Technologies). .. As for the expression vector mentioned above, pcDNA3.1/V5/His empty vector (Invitrogen) was used.

    Transfection:

    Article Title: Clock gene BMAL2
    Article Snippet: .. Human embryonic kidney 293EBNA cells (Invitrogen) were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (Life Technologies) which cultured cells were then plated at 3×105 cells per well on six-well plates and transfected by using a total of 1.0 μg of various expression plasmids [an expression vector, plasmids containing reporter genes, 0.25 ng of Renilla luciferase reporter (pRL-CMV; Promega), and plasmids containing cDNA of each clock gene (cBmal1, cBmal2, cClock) with the amount indicated in FIG. 16] together with Lipofectamine plus (Life Technologies). .. As for the expression vector mentioned above, pcDNA3.1/V5/His empty vector (Invitrogen) was used.

    Expressing:

    Article Title: Clock gene BMAL2
    Article Snippet: .. Human embryonic kidney 293EBNA cells (Invitrogen) were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (Life Technologies) which cultured cells were then plated at 3×105 cells per well on six-well plates and transfected by using a total of 1.0 μg of various expression plasmids [an expression vector, plasmids containing reporter genes, 0.25 ng of Renilla luciferase reporter (pRL-CMV; Promega), and plasmids containing cDNA of each clock gene (cBmal1, cBmal2, cClock) with the amount indicated in FIG. 16] together with Lipofectamine plus (Life Technologies). .. As for the expression vector mentioned above, pcDNA3.1/V5/His empty vector (Invitrogen) was used.

    Luciferase:

    Article Title: Clock gene BMAL2
    Article Snippet: .. Human embryonic kidney 293EBNA cells (Invitrogen) were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (Life Technologies) which cultured cells were then plated at 3×105 cells per well on six-well plates and transfected by using a total of 1.0 μg of various expression plasmids [an expression vector, plasmids containing reporter genes, 0.25 ng of Renilla luciferase reporter (pRL-CMV; Promega), and plasmids containing cDNA of each clock gene (cBmal1, cBmal2, cClock) with the amount indicated in FIG. 16] together with Lipofectamine plus (Life Technologies). .. As for the expression vector mentioned above, pcDNA3.1/V5/His empty vector (Invitrogen) was used.



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